Novel hepatocyte encapsulation enhances cellular functions

Author

Ser-Mien Chia ; Li, Jun ; Xu, Xi ; Gao, Shujun ; Leong, Kam W. ; Yu, Hanry

Author_Institution

Med. Inst., Nat. Univ. of Singapore, Singapore

Volume

2

fYear

1999

fDate

36434

Abstract

We have synthesized a HEMA, MMA and MAA terpolymer to complex with modified collagen for encapsulation of rat hepatocytes. The encapsulated hepatocytes exhibited high level of functions in culture comparable to those of hepatocyte spheroids. The collagen that lined the inner layer of the capsule provided a compatible substrate for hepatocyte culture, and the outer terpolymer shell determined permeability, which was optimized for transport of 60 kDa but not 150 kDa molecules. Besides shorter processing time than spheroid formation, the encapsulation may offer other advantages important in designing a bioartificial liver-assisted device

Keywords

biological specimen preparation; biomedical materials; cellular transport; encapsulation; liver; polymer blends; proteins; bioartificial liver-assisted device; compatible substrate; complex with modified collagen; enhanced cellular function; hepatocyte culture; hepatocyte encapsulation; rat hepatocytes; semi-permeability; terpolymer synthesis; Biological materials; Biomedical engineering; Biomedical materials; Bioreactors; Cyclic redundancy check; Encapsulation; Optical fiber devices; Permeability; Polymers; Stability;

fLanguage

English

Publisher

ieee

Conference_Titel

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint

Conference_Location

Atlanta, GA

ISSN

1094-687X

Print_ISBN

0-7803-5674-8

Type

conf

DOI

10.1109/IEMBS.1999.803877

Filename

803877