Title :
Studies of transport limitations in the dextran layer of the BIACORE optical biosensor
Author :
Sikavitsas, Vassilios I. ; Nitsche, Johannes M. ; Mountziaris, T.J. ; Daiss, John L.
Author_Institution :
Dept. of Chem. Eng., State Univ. of New York, Buffalo, NY, USA
Abstract :
Surface plasmon resonance based biosensors have become increasingly popular for the measurement of the kinetic constants in a wide variety of biomolecular interactions. One of the most popular biosensors based on this principle is the BIACORE instrument. A mathematical model is presented describing biomolecular interactions in the flowcell of the BIACORE. The model accounts not only for mass transport limitations due to the concentration gradient directly above the sensor´s dextran hydrogel matrix, but also for the effects of hindered transport of the mobile reactants into the dextran hydrogel matrix while diffusing towards their immobilized counterparts. Experiments have been also conducted on a BIACORE 2000 instrument that validate the proposed model
Keywords :
binding energy; biochemistry; biosensors; chemical sensors; mass transfer; optical sensors; reaction rate constants; refractometers; surface chemistry; surface plasmon resonance; BIACORE optical biosensor; biomolecular interactions; concentration gradient; dextran hydrogel matrix; dextran layer; hindered transport; kinetic constants; ligand binding; mass transport limitations; mathematical model; reaction rate constants; refractive index changes; sensor flow cell; surface plasmon resonance based biosensors; surface reaction rates; Biochemical analysis; Biomembranes; Biosensors; Genetic mutations; Least squares methods; Plasmons; Proteins; Resonance;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.803963
