Measurement of genetic instability in breast cancer by confocal microscopy and 3D image analysis

Author

De Solorzano, C. Ortiz ; Chin, K. ; Chou, W.S. ; Knowles, D. ; Gray, J.W. ; Lockett, S.J.

Author_Institution

Life Sci. Div., Lawrence Berkeley Nat. Lab., CA, USA

Volume

2

fYear

1999

fDate

36434

Abstract

Genetic instability (GI) is frequently associated with solid tumors including cancers of the breast. We hypothesize that the degree of GI, as evidenced by the cell-to-cell variations in the copy number of specific DNA loci, can be used as a marker for cancer prognosis. We present a computer-aided method for GI measurement in thick tissue sections based on confocal microscopy and 3D image analysis and visualization. The results, based on the analysis of breast tissue sections show considerable cell-to-cell variability in the copy number of the centromere of chromosome 1 and the 20q13 locus in invasive cancer but the normal 2 copies per cell in histologically normal tissue

Keywords

DNA; cancer; cellular biophysics; genetics; medical image processing; molecular biophysics; optical microscopy; tumours; breast cancer; breast tissue sections; cancer prognosis; cell-to-cell variability; cell-to-cell variations; centromere; chromosome 1; computer-aided method; confocal microscopy; copy number; genetic instability; histologically normal tissue; invasive cancer; solid tumors; specific DNA loci; thick tissue sections; three dimensional image analysis; visualization; Breast cancer; Breast neoplasms; Breast tissue; DNA; Genetics; Image analysis; Microscopy; Solids; Thickness measurement; Visualization;

fLanguage

English

Publisher

ieee

Conference_Titel

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint

Conference_Location

Atlanta, GA

ISSN

1094-687X

Print_ISBN

0-7803-5674-8

Type

conf

DOI

10.1109/IEMBS.1999.803977

Filename

803977