Title :
Micro fluidic single embryo culture systems in PDMS
Author :
Zeringue, H.C. ; Glasgow, I.K. ; Beebe, D.J. ; Lyman, J.T. ; Wheeler, M.B.
Author_Institution :
Beckman Inst. for Adv. Sci. & Technol., Urbana, IL, USA
Abstract :
We have designed, built and tested a micro fluidic system from poly(dimethylsiloxane) capable of culturing individual, preimplantation mouse embryos. The embryos are held stationary in “parking places” while media flows over them. The channels are 200 μm deep and 200 μm wide
Keywords :
biological techniques; microfluidics; moulding; polymers; veterinary medicine; 200 micron; microchannels; microfluidic system; poly(dimethylsiloxane) mold; preimplantation mouse embryos; single embryo culture systems; viability; Animals; Biological materials; Design engineering; Embryo; Fabrication; Fluidic microsystems; Mice; Microfluidics; Nuclear magnetic resonance; Spectroscopy;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.804006
