Title :
Electrical impedance based monitoring of viscous drag of living cells
Author :
Collins, J. ; Lavigne, D. ; Lee, A.P.
Author_Institution :
Dept. of Biomed. Eng., California Univ., Irvine, CA
fDate :
Oct. 30 2005-Nov. 3 2005
Abstract :
Electrical impedance based detection of the settling of living cells in wells equipped with microelectrode array is demonstrated in this paper. The experiment is conducted in a constant temperature incubator at 37degC with two wells of cells, one well being the experimental well and the other being the control. The impedances of the cells are generated continuously after dropping the cells at a constant density in both the wells. It takes 30 minutes to 1 hr to reach constant temperature before the experiment for cell settling measurement is started. The cells are stirred in the media of the experimental well using micropipette aspiration or using acoustic cavitation streaming micromixer and the impedance measurements are performed at high sampling rate. The ratio of the impedances for both the wells is the measure for analyzing the viscous drag of the cells experience by the media
Keywords :
bioelectric phenomena; biological techniques; cellular transport; drag; electric impedance measurement; microelectrodes; 30 to 60 min; 37 C; acoustic cavitation streaming micromixer; cell settling measurement; constant temperature incubator; electrical impedance; living cells; microelectrode array; micropipette aspiration; viscous drag; Acoustic measurements; Adhesives; Biomedical measurements; Current measurement; Elasticity; Electrodes; Impedance measurement; Magnetic resonance; Monitoring; Viscosity;
Conference_Titel :
Sensors, 2005 IEEE
Conference_Location :
Irvine, CA
Print_ISBN :
0-7803-9056-3
DOI :
10.1109/ICSENS.2005.1597911
