A real-time Linux based laser trapping system

Author

Gaffney, Donald P. ; Kennedy, Guy G. ; Warshaw, David M.

Author_Institution

Dept. of Molecular Physiol. & Biophys., Vermont Univ., Burlington, VT, USA

Volume

2

fYear

1999

fDate

36434

Abstract

A laser trapping system for the study of actomyosin interactions at the single molecule level has been developed using Real-Time Linux (RT-Linux). The laser trap has been successfully employed to examine both displacements and near-isometric forces generated by a single myosin molecular motor interacting with an actin filament. In these assays an actin filament is strung between two beads that may be manipulated in solution by infrared laser traps. It is concluded that RT-Linux has proven to be capable of providing the required timing control as well as accommodating control of other components and data acquisition

Keywords

biological techniques; measurement by laser beam; molecular biophysics; proteins; radiation pressure; actomyosin interactions; biophysical research technique; data acquisition; infrared laser traps; near-isometric forces; real-time Linux based laser trapping system; timing control; Linux; Real time systems;

fLanguage

English

Publisher

ieee

Conference_Titel

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint

Conference_Location

Atlanta, GA

ISSN

1094-687X

Print_ISBN

0-7803-5674-8

Type

conf

DOI

10.1109/IEMBS.1999.804030

Filename

804030