Three-dimensional fluorescence microscopy in two-photon excitation regime

Author

Diaspro, A. ; Corosu, M. ; Ramoino, P. ; Rotello, M.

Author_Institution

Dept. of Phys., Genoa Univ., Italy

Volume

2

fYear

1999

fDate

36434

Abstract

In our laboratory, within the framework of a national INFM project, we have realised a two-photon excitation fluorescence microscope part of a multipurpose architecture including also lifetime imaging and fluorescence correlation spectroscopy modules. The core of the architecture are a mode-locked Ti:sapphire laser (Tsunami 3960, Spectra Physics Inc., CA) pumped by a high-power (5 W, 532 nm) laser (Millennia V, Spectra Physics Inc., CA) and an ultracompact scanning head, Nikon PCM2000 (Nikon Instr., Italy). Three-dimensional confocal images of fluorophores excitable in UV and the blue region are shown. We are currently characterising the architecture in terms of point-spread-function and biological system viability

Keywords

biological techniques; fluorescence spectroscopy; optical microscopy; optical transfer function; radiative lifetimes; two-photon spectroscopy; 5 W; 532 nm; Al₂O₃:Ti; UV region; biological system viability; blue region; fluorescence correlation spectroscopy module; fluorophores; high-power laser pump; lifetime imaging; mode-locked Ti:sapphire laser; multipurpose architecture; national INFM project; point-spread-function; three-dimensional confocal images; three-dimensional fluorescence microscopy; two-photon excitation fluorescence microscope; two-photon excitation regime; ultracompact scanning head; Fluorescence; Laboratories; Laser excitation; Laser mode locking; Laser theory; Microscopy; Physics; Pump lasers; Spectroscopy; Tsunami;

fLanguage

English

Publisher

ieee

Conference_Titel

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint

Conference_Location

Atlanta, GA

ISSN

1094-687X

Print_ISBN

0-7803-5674-8

Type

conf

DOI

10.1109/IEMBS.1999.804254

Filename

804254