Title :
Optical scatter imaging of hippocampal brain slices
Author :
Johnson, L. ; Chung, W. ; Hanley, D. ; Thakor, N.
Author_Institution :
Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA
Abstract :
The mechanism of the brain´s response to injury resulting from stroke or edema is studied by optical imaging of in vitro brain slice preparations. Optical transmission as well as scatter imaging are employed to discriminate regional morphological changes in hippocampal slices. A novel imaging system based on Mie scatter theory is described. The results show that dual angle light scattering distinguishes between the cellular swelling response due to hypotonic (edema model) or N-methyl-D-apartate (stroke model) injury
Keywords :
bio-optics; biomedical imaging; brain; cellular biophysics; light scattering; light transmission; optical images; Mie scatter theory; N-methyl-D-apartate; brain response; cellular swelling response; dual angle light scattering; edema; hippocampal brain slices; hypotonic edema model; in vitro brain slice preparations; injury; optical scatter imaging; optical transmission; regional morphological changes; stroke; stroke model; Biomedical imaging; Biomedical optical imaging; Brain; Injuries; Light scattering; Mie scattering; Optical imaging; Optical refraction; Optical scattering; Particle scattering;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.804258
