Technical Advances in Spin Labelling EPR

EPR-Techniques have experienced some kind of revival and increased interest, especially in connection with biological and biomedical applications of EPR. The main aim is to get information on the localization of the spin label and its interaction with the substrate molecule. CW-saturation experiments as well as pulse techniques like saturation-recovery-ELDOR and dead-time-free double electron-electron resonance can be used for this purpose. All these techniques require some special hardware development in addition to our routine and research EPR spectrometers. One of the most promising applications of EPR imaging in oximetry.