DocumentCode
3317371
Title
Notice of Retraction
Homology Modeling and Expression Analysis of Sterol 14alpha-Demethylase from Ustilago maydis
Author
Jianhua Zhang ; Shuxiang Li ; Rui Han ; Qian Li ; Lingfu Deng ; Jinlong Wang ; Yongze Yuan ; Deli Liu
Author_Institution
Hubei Key Lab. of Genetic Regul. & Integrative Biol., Central China Normal Univ., Wuhan, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
4
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Corn smut is one of the most serious diseases affecting corn crops caused by Ustilago maydis. Sterol 14α-demethylase (CYP51) is one of the key enzymes of sterol biosynthesis and has been shown to be an effective target of antifungal drugs. To design novel antifungal compounds against UmCYP51, in this study, homology modeling and expression of CYP51 from U. maydis (UmCYP51) were carried out. A-three-dimensional structure model of UmCYP51 was built through homology modeling based on the crystal structure of Mycobacterium tuberculosis. Only pET32-Um-35 could be highly expressed in E. coli BL21 (DE3)successfully after many attempts, which indicated N terminal transmembrane regions and the lowest energy of mRNA structure are importment for the protein expression of UmCYP51, combining of bioinformatics analysis of transmembrane prediction and mRNA secondary structure of translation initiation region. These results will provide some information for predicting the expression of protein from the angle of bioinformatics, and will set the stage for insighting the feature of the UmCYP51.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Corn smut is one of the most serious diseases affecting corn crops caused by Ustilago maydis. Sterol 14α-demethylase (CYP51) is one of the key enzymes of sterol biosynthesis and has been shown to be an effective target of antifungal drugs. To design novel antifungal compounds against UmCYP51, in this study, homology modeling and expression of CYP51 from U. maydis (UmCYP51) were carried out. A-three-dimensional structure model of UmCYP51 was built through homology modeling based on the crystal structure of Mycobacterium tuberculosis. Only pET32-Um-35 could be highly expressed in E. coli BL21 (DE3)successfully after many attempts, which indicated N terminal transmembrane regions and the lowest energy of mRNA structure are importment for the protein expression of UmCYP51, combining of bioinformatics analysis of transmembrane prediction and mRNA secondary structure of translation initiation region. These results will provide some information for predicting the expression of protein from the angle of bioinformatics, and will set the stage for insighting the feature of the UmCYP51.
Keywords
bioinformatics; biomembranes; botany; crops; crystal structure; diseases; enzymes; microorganisms; molecular biophysics; CYP51; E. coli BL21; Mycobacterium tuberculosis; N terminal transmembrane regions; Sterol 14α-demethylase; UmCYP51; Ustilago maydis; antifungal drugs; bioinformatics; corn crops; corn smut; crystal structure; diseases; enzymes; homology modeling; mRNA secondary structure; pET32-Um-35; protein expression; sterol biosynthesis; translation initiation region; Analytical models; Crystals; Proteins; Solid modeling; X-ray diffraction;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5779993
Filename
5779993
Link To Document