Spectroscopic Studies on the Interaction between Bovine Serum Albumin and Syringic Acid

Author

Tang, Zhuxing ; Wang, Qiaoling ; Zhao, Zhenchuan

Author_Institution

Sch. of Environ. & Chem. Eng., Shenyang Ligong Univ., Shenyang, China

fYear

2011

fDate

10-12 May 2011

Firstpage

1

Lastpage

4

Abstract

In this paper, the interaction mechanism between bovine serum albumin (BSA) and syringic acid was investigated by using fluorescence spectroscopy, UV absorption spectra at pH=7.40 and different temperatures. The effects of temperature on the binding reaction was observed and the binding constants and the number of bonding sites were obtained. By using synchronous fluorescence analysis, syringic acid was also discussed on the effect of the conformation of BSA and the mechanism of interaction. In the molecular level, we study the binding mode between bovine serum albumin, and syringic acid.

Keywords

biochemistry; bonds (chemical); fluorescence; molecular biophysics; molecular configurations; pH; proteins; ultraviolet spectra; UV absorption spectra; binding constants; binding reaction; bonding sites; bovine serum albumin; conformation; fluorescence spectroscopy; interaction mechanism; pH; synchronous fluorescence analysis; syringic acid; Absorption; Amino acids; Bovine; Fluorescence; Plasmas; Proteins; Spectroscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on

Conference_Location

Wuhan

ISSN

2151-7614

Print_ISBN

978-1-4244-5088-6

Type

conf

DOI

10.1109/icbbe.2011.5780023

Filename

5780023