Title :
Spectroscopic Studies on the Interaction between Bovine Serum Albumin and Syringic Acid
Author :
Tang, Zhuxing ; Wang, Qiaoling ; Zhao, Zhenchuan
Author_Institution :
Sch. of Environ. & Chem. Eng., Shenyang Ligong Univ., Shenyang, China
Abstract :
In this paper, the interaction mechanism between bovine serum albumin (BSA) and syringic acid was investigated by using fluorescence spectroscopy, UV absorption spectra at pH=7.40 and different temperatures. The effects of temperature on the binding reaction was observed and the binding constants and the number of bonding sites were obtained. By using synchronous fluorescence analysis, syringic acid was also discussed on the effect of the conformation of BSA and the mechanism of interaction. In the molecular level, we study the binding mode between bovine serum albumin, and syringic acid.
Keywords :
biochemistry; bonds (chemical); fluorescence; molecular biophysics; molecular configurations; pH; proteins; ultraviolet spectra; UV absorption spectra; binding constants; binding reaction; bonding sites; bovine serum albumin; conformation; fluorescence spectroscopy; interaction mechanism; pH; synchronous fluorescence analysis; syringic acid; Absorption; Amino acids; Bovine; Fluorescence; Plasmas; Proteins; Spectroscopy;
Conference_Titel :
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location :
Wuhan
Print_ISBN :
978-1-4244-5088-6
DOI :
10.1109/icbbe.2011.5780023
