The Construction, Bioinformatic Analysis and Vitro Expression of Multi-Epitope Based Gene from Toxoplasma gondii

The search for an effective vaccine against toxoplasmosis remains a challenging and elusive goal. Combination of epitopes from different stages of T. gondii life cycle is an optimal strategy to overcome the antigen complexity of the parasite. Based on published epitope derived from several promising candidate vaccine antigens and the help of epitope predicting and protein analyzing software, we construct a multi-epitope DNA encoding multi-epitope of T. gondii. The Bioinformatic analysis was used to predict the protein structure and characteristic for T. gondii multi-epitope gene encoding protein. The compound gene was expressed in vitro, the protein could be recognized by the T. gondii antibody. The results from this study should contribute to the development of a highly protective multi-epitope DNA vaccine against T. gondii.