Title :
Notice of Retraction
Development of a Fluorescent Quantitative Real-Time Polymerase Chain Reaction Assay for the Detection of HSL Expression In Vitro
Author :
Zuo-Hua Liu ; Jin-Long Yang ; Fei-Yun Yang ; Kang-Cheng Pan ; Dai-Wen Chen
Author_Institution :
Chongqing Acad. of Animal Sci., Chongqing, China
Abstract :
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Background: Obesity results from an imbalance between energy intake and energy expenditure, which leads to a pathological accumulation of adipose tissue, but the underlying mechanism at gene level is far from being elucidated. Methods: Pig primary adipocytes were cultured in vitro and the expression of HSL was detected by real-time PCR Results: The high concentrations of glucose group significantly cut down the mRNA expression of HSL (P<;0.01) rather than in the others. Conclusion: Our results provide significant data for that confirm an association between different glucose level and HSL expression in preadipocytes.
Keywords :
DNA; association; biochemistry; biological tissues; cellular biophysics; diseases; enzymes; fluorescence; molecular biophysics; HSL expression; adipocytes; adipose tissue; association; cell culture; energy expenditure; energy intake imbalance; fluorescent quantitative real-time polymerase chain reaction assay; gene level; glucose group; glucose level; mRNA expression; obesity; pathological accumulation; real-time PCR; Fluorescence; Gene expression; Humans; In vitro; Obesity; Real time systems; Sugar;
Conference_Titel :
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location :
Wuhan
Print_ISBN :
978-1-4244-5088-6
DOI :
10.1109/icbbe.2011.5780053
