Batch adsorption of whey protein onto anion exchange mixed matrix membrane chromatography

Author

Saufi, Syed M. ; Fee, Conan J.

Author_Institution

Fac. of Chem. & Natural Resource Eng., Univ. Malaysia Pahang, Kuantan, Malaysia

fYear

2010

fDate

2-4 Nov. 2010

Firstpage

23

Lastpage

25

Abstract

Mixed matrix membrane (MMM) chromatography is an advanced method for whey protein fractionation. The adsorption capacity of MP500 anion exchange MMM chromatography was evaluated by batch experiments using simulated whey containing a single whey protein component and real whey at pH 6. The binding preference for whey proteins was demonstrated to follow the order: β-lactoglobulin > bovine serum albumin > α-lactalbumin. MP500 based MMM could be used to recover β-lactoglobulin very selectively from whey to produce a single protein component for food ingredient and related applications.

Keywords

adsorption; batch processing (industrial); chemical engineering; chromatography; food processing industry; fractionation; ion exchange; membranes; proteins; α-lactalbumin; β-lactoglobulin; adsorption capacity; anion exchange mixed matrix membrane chromatography; batch adsorption; bovine serum albumin; food ingredient; protein fractionation; whey protein; Proteins; anion exchange membrane; component; membrane chromatography; whey protein;

fLanguage

English

Publisher

ieee

Conference_Titel

Chemical, Biological and Environmental Engineering (ICBEE), 2010 2nd International Conference on

Conference_Location

Cairo

Print_ISBN

978-1-4244-8748-6

Electronic_ISBN

978-1-4244-8749-3

Type

conf

DOI

10.1109/ICBEE.2010.5650595

Filename

5650595