Title :
Batch adsorption of whey protein onto anion exchange mixed matrix membrane chromatography
Author :
Saufi, Syed M. ; Fee, Conan J.
Author_Institution :
Fac. of Chem. & Natural Resource Eng., Univ. Malaysia Pahang, Kuantan, Malaysia
Abstract :
Mixed matrix membrane (MMM) chromatography is an advanced method for whey protein fractionation. The adsorption capacity of MP500 anion exchange MMM chromatography was evaluated by batch experiments using simulated whey containing a single whey protein component and real whey at pH 6. The binding preference for whey proteins was demonstrated to follow the order: β-lactoglobulin > bovine serum albumin > α-lactalbumin. MP500 based MMM could be used to recover β-lactoglobulin very selectively from whey to produce a single protein component for food ingredient and related applications.
Keywords :
adsorption; batch processing (industrial); chemical engineering; chromatography; food processing industry; fractionation; ion exchange; membranes; proteins; α-lactalbumin; β-lactoglobulin; adsorption capacity; anion exchange mixed matrix membrane chromatography; batch adsorption; bovine serum albumin; food ingredient; protein fractionation; whey protein; Proteins; anion exchange membrane; component; membrane chromatography; whey protein;
Conference_Titel :
Chemical, Biological and Environmental Engineering (ICBEE), 2010 2nd International Conference on
Conference_Location :
Cairo
Print_ISBN :
978-1-4244-8748-6
Electronic_ISBN :
978-1-4244-8749-3
DOI :
10.1109/ICBEE.2010.5650595
