DocumentCode :
3320183
Title :
Method of mRNA Display for Selecting Peptides That Bind with Target Enzyme RNA
Author :
Yan Song ; Wang Zheng ; Yang Shao-li ; Zhang Yi ; Lin Xiu-kun
Author_Institution :
Key Lab. of Exp. Marine Biol., Chinese Acad. of Sci., Qingdao, China
fYear :
2011
fDate :
10-12 May 2011
Firstpage :
1
Lastpage :
4
Abstract :
In vitro display techniques are useful tools for selecting novel ligands that bind to target biomolecules. In this paper, mRNA display method was used to select peptides that bind thymidylate synthase RNA with high affinity from a peptide library. We provide a detailed analysis of the critical steps of an mRNA-display-based selection, including the generation of an mRNA-displayed library, formation of RNA-peptide fusions, and selection of RNA affinity peptides. After optimization of the experimental steps, the efficiency of RNA-linker purification and the reverse transcription was Improved, and the unspecified binding peptides were removed. Our study could facilitate the use of mRNA display in the field of biotechnology, medicine and proteomics.
Keywords :
DNA; biochemistry; biological techniques; bonds (chemical); enzymes; molecular biophysics; optimisation; DNA sequences; PCR amplification; RNA-linker purification; RNA-peptide fusions; biomolecules; biotechnology; in-vitro display techniques; mRNA display; medicine; molecular binding; optimization; peptide selection; proteomics; reverse transcription; target enzyme RNA; thymidylate synthase RNA; Cloning; DNA; In vitro; Libraries; Peptides; Proteins; RNA;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location :
Wuhan
ISSN :
2151-7614
Print_ISBN :
978-1-4244-5088-6
Type :
conf
DOI :
10.1109/icbbe.2011.5780178
Filename :
5780178
Link To Document :
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