Investigation of Phage as biosensitive film for LAPS

Author

Jia, Yunfang ; Niu, Wencheng ; Li, Xiao ; Zhang, Fuhai ; Liu, Guohua ; Zhang, Hongkai ; Bai, Yunpeng ; Cao, Youjia ; Qin, Ming

Author_Institution

Univ. of Nankai, Tianjin

fYear

2007

fDate

8-11 July 2007

Firstpage

52

Lastpage

57

Abstract

Here the phage probe as the bio-sensitive film for Light Addressable Potentiometric Sensor (LAPS) is studied. The N-terminal of the phage probes were covalently fixed on the surface of LAPS. Using the back-illuminating system the phage- LAPSs were tested to detect Human Phosphatase of Regenerating Liver-3 (hPRL-3) and the mammary adenocarcinoma cell (MDA-MB231) in the PBS solutions and treated blood solutions. The sensitivities in PBS were about - 3.33 muV/p[hPRL-3] and 15muV/p[MDA-MB231], respectively. And changes were found in different solutions. Discusions are given to these differences.

Keywords

biomedical equipment; biomedical measurement; biosensors; potentiometers; probes; back-illuminating system; biosensitive film; blood solutions; human phosphatase of regenerating liver-3; light addressable potentiometric sensor; phage probe; Biosensors; Cancer; Cells (biology); Cities and towns; Costs; FETs; Humans; Immune system; Metastasis; Probes; Cell detection; LAPS; Phage display;

fLanguage

English

Publisher

ieee

Conference_Titel

Information Acquisition, 2007. ICIA '07. International Conference on

Conference_Location

Seogwipo-si

Print_ISBN

1-4244-1220-X

Electronic_ISBN

1-4244-1220-X

Type

conf

DOI

10.1109/ICIA.2007.4295696

Filename

4295696