DocumentCode
3322165
Title
Notice of Retraction
Comparison of the Biological Characteristics between Diabetes and Normal Skin Derived Epidermal Stem Cells In Vitro
Author
Feibin Zhu ; Dewu Liu ; Hongyan Zhang ; Yan Peng ; Qingling Zhong ; Yongtie Li ; Pu Ning
Author_Institution
Burns Inst., First Affiliated Hosp. of Nanchang Univ., Nanchang, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
4
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Epidermal stem cells (ESCs) play a critical roles in wound healing. But the biological characteristics of ESCs derived from diabetes mellitus are not fully understood. In this chapter, we compared the biological characteristics between diabetes and normal skin derived ESCs in vitro. The diabetes mellitus (DM) rat models were made by intraperitoneal injected streptozotocin. ESCs were extracted from the dorsal skin samples of rats by trypsin digesting method and further purified by collagen adhering methods. The morphologic change and cell colony formation were observed. The expression of K19, β1-integrin were identified by immunocytochemical stain and image analysis. The results showed that the number of primary anchorage-dependent ESCs in DM group was decreased. The clone forming efficiency of ESCs in DM group was significantly lower than that in the normal control group. ESCs of two groups both express K19, P 1-integrin. The integral absorbance values of positive cells for K19, β1-integrin in ESCs of DM group were significantly lower than those in the normal control group. This suggested that the multiplication capacity in vitro of rat ESCs derived from diabetes skin was lower than that in normal skin, which may be one of important factors that diabetes mellitus wound is hard to heal.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Epidermal stem cells (ESCs) play a critical roles in wound healing. But the biological characteristics of ESCs derived from diabetes mellitus are not fully understood. In this chapter, we compared the biological characteristics between diabetes and normal skin derived ESCs in vitro. The diabetes mellitus (DM) rat models were made by intraperitoneal injected streptozotocin. ESCs were extracted from the dorsal skin samples of rats by trypsin digesting method and further purified by collagen adhering methods. The morphologic change and cell colony formation were observed. The expression of K19, β1-integrin were identified by immunocytochemical stain and image analysis. The results showed that the number of primary anchorage-dependent ESCs in DM group was decreased. The clone forming efficiency of ESCs in DM group was significantly lower than that in the normal control group. ESCs of two groups both express K19, P 1-integrin. The integral absorbance values of positive cells for K19, β1-integrin in ESCs of DM group were significantly lower than those in the normal control group. This suggested that the multiplication capacity in vitro of rat ESCs derived from diabetes skin was lower than that in normal skin, which may be one of important factors that diabetes mellitus wound is hard to heal.
Keywords
biomedical measurement; cellular biophysics; diseases; molecular biophysics; proteins; skin; wounds; β1-integrin expression; K19 expression; biological characteristics; cell colony formation; cell integral absorbance; clone forming efficiency ESC; collagen adhering methods; diabetes mellitus rat models; diabetic skin derived ESC; dorsal skin; epidermal stem cells; image analysis; immunocytochemical stain; morphologic change; normal skin derived ESC; primary anchorage dependent ESC; streptozotocin intraperitoneal injection; trypsin digesting method; wound healing; Cloning; Diabetes; Epidermis; Rats; Stem cells; Wounds;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5780281
Filename
5780281
Link To Document