DocumentCode
3322217
Title
Notice of Retraction
Using RAPD to Analyze Genetic Stability of Populus hopeiensis Tissue Culture Seeding by Different Regeneration Methods
Author
Qiong Zhang ; Gang Liu
Author_Institution
Biotechnol. Res. Center, China Three Gorges Univ., Yichang, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
3
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
RAPD markers were used to analyze genetic stability of regenerated plant from different ways of Populus hopeiensis in this study. The results indicated that, using the young leaves of vitro plantlets as explants, the RAPD bands were same between the control and regenerated plant which was from the direct regenerating adventitious buds. The young leaves of vitro plantlets were as explants to induce the callus, and there was a polymorphic band respectively in the primer S268 and S104 on 2 regenerated plants which were from the way of organogenesis. The mutational rate of RAPD sites and plants were 2/288 and 2/20 respectively. It indicated that there was somaclonal variation during training by this way, and the genetic stability was lower than by the former way.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
RAPD markers were used to analyze genetic stability of regenerated plant from different ways of Populus hopeiensis in this study. The results indicated that, using the young leaves of vitro plantlets as explants, the RAPD bands were same between the control and regenerated plant which was from the direct regenerating adventitious buds. The young leaves of vitro plantlets were as explants to induce the callus, and there was a polymorphic band respectively in the primer S268 and S104 on 2 regenerated plants which were from the way of organogenesis. The mutational rate of RAPD sites and plants were 2/288 and 2/20 respectively. It indicated that there was somaclonal variation during training by this way, and the genetic stability was lower than by the former way.
Keywords
DNA; biological techniques; botany; genetics; molecular biophysics; Populus hopeiensis; RAPD bands; RAPD markers; RAPD site mutational rate; callus; direct regenerating adventitious buds; genetic stability analysis; organogenesis; polymorphic band; random amplification of polymorphic DNA; regenerated plant; regeneration methods; somaclonal variation; tissue culture seeding; DNA; Materials; Soil; Stability analysis; Thermal stability; Vegetation;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5780284
Filename
5780284
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