Title :
Design of an In Situ Process Analyzer Instrument for Organism Measurement Underwater
Author :
Lou, Kai-kai ; Liu, Yu-jing ; Ye, Shu-ming ; Chen, Hang
Author_Institution :
Dept. of Biomed. Eng., Zhejiang Univ., Hangzhou, China
Abstract :
Research of the quantity of microorganism in the deep sea is of great importance for the development of ecosystem science. Because the marine ecosystem is a complicated system that varies anytime and anywhere, an in situ microorganism measurement device is indispensable. The adenosine triphosphate (ATP) can only be produced in the living organism. With ATP, the fluorescein in microorganism can be catalyzed by luciferase to generate fluorescence. Basing on the ATP bioluminescence reaction detection principle, an in-situ process analyzer instrument for organism detection underwater is presented.
Keywords :
biochemistry; bioluminescence; biosensors; catalysis; cellular biophysics; ecology; enzymes; fluorescence; microorganisms; molecular biophysics; oceanographic equipment; oceanographic techniques; underwater equipment; ATP bioluminescence reaction detection; ATP molecules; adenosine triphosphate; catalysis; deep-sea research; fluorescein; fluorescence; in-situ process analyzer instrument; luciferase; marine ecosystem; microbial biomass; microorganism quantity; underwater organism measurement; Bioluminescence; Chemicals; Fluorescence; Instruments; Organisms; Sea measurements; Valves;
Conference_Titel :
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location :
Wuhan
Print_ISBN :
978-1-4244-5088-6
DOI :
10.1109/icbbe.2011.5780332