Notice of RetractionStudy on Effects of N-Butanol Extraction Layer of Geranium Wilfordii on Apoptosis of HepG2 Cells

Notice of Retraction

After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.

We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.

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Objective: To investigate the inhibition of n-butanol extraction layer of Geranium Wilfordii on proliferation of human liver cancer HepG₂ cells and its function mechanism. Methods: Different concentrations of n-butanol extraction layers of Geranium Wilfordii were used to affect HepG₂ cells cultured in vitro. SRB assay was used to determine inhibition rate of cell growth, and flow cytometry assay was used to determine apoptosis. Fluorescent probe Fluo-3/AM was used to observe changes of concentration of intracellular calcium ion of tumor cells, and flow cytometry was used to determine changes of intracellular active oxygen species of tumor cells. Results: n-butanol extraction layer could significantly inhibit growth of HepG₂ cells and induce occurrence of apoptosis, and the IC50 was 83.51 ug/ml. After effects of n-butanol extraction layer for 48h, the amount of HepG₂ cells significantly reduced. Under the effect of Fluo-3/AM fluorescent probe, stronger green fluorescence appeared. At the same time, detected intracellular ROS more significantly increased. Conclusion: n-butanol layer of Geranium Wilfordii could inhibit growth of HepG₂ cells and induce occurrence of apoptosis, which was possibly realized by means of the release of intracellular Ca²⁺ ion of HepG₂ cells and the increase of intracellular active oxygen species.