Cell dynamics under cold plasma treatment

Author

Volotskova, O. ; Shashurin, A. ; Keidar, M. ; Stepp, M.A.

Author_Institution

MAE Dept., George Washington Univ., Washington, DC, USA

fYear

2010

fDate

20-24 June 2010

Firstpage

1

Lastpage

1

Abstract

Summary form only given. Cold plasmas with ion temperatures close to room temperatures are one of the promising and challenging areas of research. Their unique properties allow using them in various areas including medicine and biology, providing tool for living tissue treatment. This report is focused on the studying of helium cold jet and its interaction with living cells. Living cells (tertiary wild type fibroblast) are treated with helium plasma jet and its influence on the migration rates are studied including temporal behavior of cells after treatment. Localization of the effect of plasma jet on cell dynamics is investigated. Cell tracking is performed during 32 hours in three localized areas (treated area, near treated area and far from treated area). Fixed and stained cells were studied using fluorescence microscopy to identify changes in cell skeleton and focal adhesions. Helium plasma jet is characterized by UV-vis-NIR spectrum in both transverse and axial directions.

Keywords

biological tissues; cell motility; helium; plasma applications; plasma jets; plasma temperature; He; NIR spectrum; UV-spectrum; cell dynamics; cell temporal behavior; cell tracking; cold plasma treatment; fluorescence microscopy; helium cold jet; ion temperature; living tissue treatment; tertiary wild type fibroblast; Biological tissues; Cells (biology); Fibroblasts; Fluorescence; Helium; Medical treatment; Microscopy; Plasma properties; Plasma temperature; Skeleton;

fLanguage

English

Publisher

ieee

Conference_Titel

Plasma Science, 2010 Abstracts IEEE International Conference on

Conference_Location

Norfolk, VA

ISSN

0730-9244

Print_ISBN

978-1-4244-5474-7

Electronic_ISBN

0730-9244

Type

conf

DOI

10.1109/PLASMA.2010.5534040

Filename

5534040