High-power EM-pulse influence on isolated cells

The influence of a RF microsecond pulse on donor erythrocytes and of a short high-power electromagnetic videopulse on a suspension of hepatic cells of 9-weeks human embryo has been investigated. Such pulse was generated by an electron beam of the accelerator "Temp-A" with energy 0.6 MeV, current 4 kA, and time duration 40 nsec under falling at rod antenna of 3 in length. The output power was 100 MW. The samples were placed perpendicular to the antenna at the distance 30 cm from its middle. The free-radical state in the system "hepatocyte+hypochlorite" has been investigated on the quantum-metrical installation with photo-electronic amplifier FEU-140 in a thermostat box at 37°C. The appearance of radical fragments in this system was registered by means of spectral line 630 nm of generated singlet oxygen. The estimation of the viability of cells before and after irradiation was performed by using the method of supravital staining by methylene dark blue. It was shown that the influence of videopulse treatment decreases the percentage of the viability of the hepatocytes and activates the free-radical processes and correspondingly enhances the singlet oxygen generation. The dependence of these processes on the videopulses amount was determined.