Title :
A multi-layer PDMS microfluidic device for tissue engineering applications
Author :
Leclerc, Eric ; Sakai, Yasuyuki ; Fujii, Teruo
Author_Institution :
CNRS, Univ. of Tokyo, Japan
Abstract :
A PDMS microfluidic device composed of several microstructured layers is fabricated for perfusion culture of mammalian cells. The device has been tested with Hep G2 and 3T3-L1 cells to investigate the possibilities of microfluidic/MEMS devices in the field of tissue engineering. Comparison between a two layer device and multi-layer devices with and without oxygen chamber has shown the importance of oxygen supply in large scale cell culture required for tissue reconstruction.
Keywords :
biological specimen preparation; biological tissues; cellular biophysics; microfluidics; micromachining; oxygen; 3T3-L1 cells; Hep G2 cells; MEMS devices; O2; large scale cell culture; mammalian cells; microfluidic devices; microstructured layers; multi-layer PDMS microfluidic device; multi-layer devices; oxygen chamber; oxygen supply; perfusion culture; tissue engineering; tissue reconstruction; two-layer device; Biological materials; Bioreactors; Bonding; Large-scale systems; Microfluidics; Oxygen; Permeability; Telephony; Testing; Tissue engineering;
Conference_Titel :
Micro Electro Mechanical Systems, 2003. MEMS-03 Kyoto. IEEE The Sixteenth Annual International Conference on
Print_ISBN :
0-7803-7744-3
DOI :
10.1109/MEMSYS.2003.1189774
