Title :
Dielectrophoretic manipulation of avidin and DNA
Author :
Bakewell, David J G ; Hughes, Michael P. ; Milner, Joel J. ; Morgan, Hywel
Author_Institution :
Bioelectron. Res. Centre, Glasgow Univ., UK
fDate :
29 Oct-1 Nov 1998
Abstract :
This paper demonstrates the application of dielectrophoresis (DEP) to the manipulation of biological macromolecules such as avidin and plasmid DNA. We report, for the first time, observations of negative dielectrophoresis of these macromolecules which can be used to perform stable trapping. The basic electrokinetic theory and experimental arrangements for realising DEP in microelectrode structures are described. The paper discusses our observations on the DEP manipulation of these macromolecules
Keywords :
Brownian motion; DNA; biological techniques; biotechnology; electrokinetic effects; electrophoresis; molecular biophysics; proteins; Brownian motion; avidin; biological macromolecules; dielectrophoretic manipulation; electrokinetic theory; microelectrode structures; negative dielectrophoresis; plasmid DNA; polyelectrolytes; stable trapping; Biology; DNA; Dielectrophoresis; Electrodes; Electrokinetics; Microelectrodes; Molecular biophysics; Nonuniform electric fields; Polarization; Voltage;
Conference_Titel :
Engineering in Medicine and Biology Society, 1998. Proceedings of the 20th Annual International Conference of the IEEE
Conference_Location :
Hong Kong
Print_ISBN :
0-7803-5164-9
DOI :
10.1109/IEMBS.1998.745640
