DNA Biosensor Based on the CdS Modified Glassy Carbon Electrode

Author

Xu, Laihui ; Lu, Liping ; Gao, Zhao ; Kang, Tianfang ; Cheng, Shuiyuan

Author_Institution

Coll. of Enviromental & Energy Eng., Beijing Univ. of Technol., Beijing, China

fYear

2011

fDate

10-12 May 2011

Firstpage

1

Lastpage

3

Abstract

An electrochemical sensor for DNA hybridization detection using methylene blue (MB) as indicator was developed. CdS nanoparticals were electrodeposited on the glassy carbon electrode (GCE). DNA probe and the CdS was linked by cysteine. The electrochemical response of the DNA hybridization was measured by differential pulse voltammetry (DPV). The differ ence of the cathodic peak currents of MB after the hybridization of the DNA probe was obvious. In addition, the morphology of the modified electrode was characterized by AFM.

Keywords

DNA; II-VI semiconductors; atomic force microscopy; biochemistry; biological techniques; biosensors; cadmium compounds; electrodeposition; molecular biophysics; nanoparticles; nanosensors; voltammetry (chemical analysis); AFM; C; CdS; DNA biosensor; DNA hybridization; DNA hybridization detection; DNA probe; cysteine; differential pulse voltammetry; electrochemical analysis; electrochemical sensor; electrodeposition; glassy carbon electrode; methylene blue; nanoparticals; Biosensors; DNA; Electrodes; Morphology; Nanoparticles; Probes; Surface morphology;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on

Conference_Location

Wuhan

ISSN

2151-7614

Print_ISBN

978-1-4244-5088-6

Type

conf

DOI

10.1109/icbbe.2011.5781421

Filename

5781421