Title :
Structural and Immunogenic Effect of Complete Antigen Linked by Different Spacer Arm
Author :
Luo, Shunjing ; Chen, Tingting ; Liu, Chengmei ; Zou, Changcun ; Chen, Chen
Author_Institution :
State Key Lab. of Food Sci. & Technol., Nanchang Univ., Nanchang, China
Abstract :
Effects of spacer arm heterology on antibody production, screening and ELISA was investigated with the help of computational molecular modeling. Three chloramphenicol (CAP) haptens with different spacer arms were conjugated with carrier proteins. Balb/c mice was immunized with complete antigen, then serum titre and affinity were detected. Theoretic geometric and electronic parameters were estimated, the similiarity between haptens and CAP were compared. A new CAP immunogen with para-methyl benzoate as spacer arm was synthesised. Spacer arm with para-oriented aromatic ring induced extreme structural change of haptens, yet improved the sensitivity of ELISA test.
Keywords :
biochemistry; biological techniques; biology computing; drugs; electronic structure; macromolecules; proteins; ELISA test; antibody production; antibody screening; chloramphenicol haptens; computational molecular modeling; electronic parameters; geometric parameters; immunogenic effect; paramethyl benzoate; paraoriented aromatic ring; spacer arm heterology; spacer-arm linked antigen; Coatings; Immune system; Joining processes; Proteins; Sensitivity;
Conference_Titel :
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location :
Wuhan
Print_ISBN :
978-1-4244-5088-6
DOI :
10.1109/icbbe.2011.5781532
