DocumentCode
3347589
Title
Notice of Retraction
Genome Cloning of Human Bocavirus (HBoV1) and Prospective Study of NP1 Gene Transcription
Author
Jingjing Li ; Yuan Wang ; Bin Sun ; Jinfeng Ouyang ; Hu Han ; Qinqin Huang ; Yongbo Yang ; Yi Li
Author_Institution
Coll. of Life Sci., Huazhong Normal Univ., Wuhan, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
3
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Human bocavirus (HBoV) is a recently discovered parvovirus which is suspected to be an etiologic agent of respiratory disease and gastrointestinal disease in humans. In this study, We detected positive samples for HBoV1 from nasopharyngeal aspirates and constructed the nearly full-length genome clone designated as pWHL-1(GenBank Acession NO. GU139423). Furthermore, using the RT-PCR and 3´RACE method we identified different viral mRNAs of NP1 gene in 293T cells transfected by pWHL-1. The mRNAs were varied due to alternative splicing and alternative polyadenylation. Our results showed that gene transcription occurred in pWHL-1 transfected cells even without inverted terminal repeats (ITRs) structure of the viral genome.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Human bocavirus (HBoV) is a recently discovered parvovirus which is suspected to be an etiologic agent of respiratory disease and gastrointestinal disease in humans. In this study, We detected positive samples for HBoV1 from nasopharyngeal aspirates and constructed the nearly full-length genome clone designated as pWHL-1(GenBank Acession NO. GU139423). Furthermore, using the RT-PCR and 3´RACE method we identified different viral mRNAs of NP1 gene in 293T cells transfected by pWHL-1. The mRNAs were varied due to alternative splicing and alternative polyadenylation. Our results showed that gene transcription occurred in pWHL-1 transfected cells even without inverted terminal repeats (ITRs) structure of the viral genome.
Keywords
DNA; cellular biophysics; diseases; genetics; genomics; macromolecules; microorganisms; molecular biophysics; pneumodynamics; 293T cell transfection; NP1 gene transcription; RT-PCR; alternative polyadenylation; alternative splicing; etiologic agent; full-length genome clone; gastrointestinal disease; genome cloning; human bocavirus; inverted terminal repeat structure; nasopharyngeal aspirates; pWHL-1; parvovirus; respiratory disease; viral genome; viral mRNA; Bioinformatics; Cloning; DNA; Diseases; Genomics; Humans; Proteins;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5781646
Filename
5781646
Link To Document