Title :
Efficient light-collection effect in fluorescence microscopy with solid immersion lens
Author :
Koyama, Koichi ; Baba, M. ; Yoshita, Masahiro ; Suemoto, T. ; Akiyama, Hidenori
Author_Institution :
Inst. for Solid State Phys., Tokyo Univ., Japan
Abstract :
Summary form only given. Improved light-collection efficiency and spatial resolution in fluorescence microscopy higher than the diffraction-limit values in the conventional methods have been simultaneously realized by means of a solid immersion lens (SIL), for which we investigate the relevant physics of the efficient light-collection effect. This technique should be very useful in the fluorescence microscopy of semiconductor nano-structures, single dye molecules and various applications in molecular biology.
Keywords :
biological techniques; fluorescence; lenses; molecular biophysics; optical microscopy; 600 nm; diffraction-limit values; fluorescence microscopy; light-collection effect; light-collection efficiency; molecular biology; semiconductor nano-structures; single dye molecules; solid immersion lens; spatial resolution; Biomedical optical imaging; Diffraction; Fluorescence; Lenses; Nanobioscience; Optical microscopy; Physics; Shape; Solid state circuits; Spatial resolution;
Conference_Titel :
Quantum Electronics and Laser Science Conference, 1999. QELS '99. Technical Digest. Summaries of Papers Presented at the
Conference_Location :
Baltimore, MD, USA
Print_ISBN :
1-55752-576-X
DOI :
10.1109/QELS.1999.807430
