Two-photon tomography [biological tissue imaging]

Author

Feng Liu

Author_Institution

Dept. of Phys., City Univ. of New York, NY, USA

fYear

1992

fDate

23-28 May 1992

Firstpage

261

Abstract

Summary form only given. Two-photon fluorescence and second harmonics are used to image subsurface structures of highly scattering biological tissues. Ultrashort laser pulses in the near-infrared spectra region were reflected by a dichroic beam splitter and then focused into tissue samples by an objective lens to generate a nonlinear optical signal. The generated nonlinear optical signal were collected by the same objective lens and passed through the bean splitter to a photomultiplier tube for detection.

Keywords

biological tissues; biomedical imaging; fluorescence spectroscopy; infrared imaging; laser applications in medicine; optical harmonic generation; optical tomography; two-photon spectroscopy; dichroic beam splitter; highly scattering biological tissues; near-infrared spectra region; nonlinear optical signal; objective lens; photomultiplier tube detection; second harmonics; subsurface structures; tissue samples; two-photon fluorescence; two-photon tomography; ultrashort laser pulses; Biological tissues; Biomedical optical imaging; Fluorescence; Lenses; Nonlinear optics; Optical imaging; Optical pulse generation; Optical scattering; Signal generators; Tomography;

fLanguage

English

Publisher

ieee

Conference_Titel

Quantum Electronics and Laser Science Conference, 1999. QELS '99. Technical Digest. Summaries of Papers Presented at the

Conference_Location

Baltimore, MD, USA

Print_ISBN

1-55752-576-X

Type

conf

DOI

10.1109/QELS.1999.807645

Filename

807645