Micro-Optical Sectioning Tomography for visualizing the mouse brainwide neuroanatomical connectivity

Neuroanatomical architecture is considered the basis for understanding brain functions and dysfunctions. Several imaging approaches have been made for brainwide mapping of neural circuits at a mesoscopic scale, including our developed Micro-Optical Sectioning Tomography (MOST) system that can provide submicron tomography of a centimeter-sized whole mouse brain. In this talk, we will clarify the unique features of the MOST in comparison to other techniques, discuss the challenges for visualizing the mouse brainwide neuroanatomical connectivity at the neurite level. Furthermore, we will present the new developed fMOST for imaging the fluorescent proteins or other fluorescent markers in the mouse brain.