Title :
Electrically stimulated NGF secretion by astroglial cells
Author :
Koyama, Sumihiro ; Haruyama, Tetsuya ; Kobatake, Eiry ; Aizawa, Masuo
Author_Institution :
Dept. of Bioeng., Tokyo Inst. of Technol., Yokohama, Japan
fDate :
31 Oct-3 Nov 1996
Abstract :
Mouse astrocytes were cultured on an optically transparent electrode (indium tin oxide/glass electrode) and subjected to an electrical sine wave of stimulation at 10 Hz for 1 h. The electrical stimulation of 0 to +0.3 V (vs. Ag/AgCl) peak to peak sine wave enhanced nerve growth factor (NGF) secretion by 6-fold. The mechanistic investigation indicate that the PKC dependent signaling pathway involved in astrocytes could be activated by the electrical stimulation
Keywords :
bioelectric phenomena; biological techniques; cellular biophysics; neurophysiology; 0 to 0.3 V; 1 h; 10 Hz; Ag-AgCl; PKC dependent signaling pathway; electrical sine wave; electrical stimulation; indium tin oxide/glass electrode; mechanistic investigation; mouse astrocytes culture; nerve growth factor; optically transparent electrode; Electric shock; Electrical stimulation; Electrodes; Fluids and secretions; Glass; Indium tin oxide; Mice; Production; Proteins; Surface morphology;
Conference_Titel :
Engineering in Medicine and Biology Society, 1996. Bridging Disciplines for Biomedicine. Proceedings of the 18th Annual International Conference of the IEEE
Conference_Location :
Amsterdam
Print_ISBN :
0-7803-3811-1
DOI :
10.1109/IEMBS.1996.646432
