Title :
Characterization of a D-Amino Acid Oxidase Microbiosensor for D-Serine Detection in the Central Nervous System
Author :
Pernot, Pierre ; Mothet, J.-P. ; Schuvailo, Oleg ; Soldatkin, Alexey ; Pollegioni, Loredano ; Pilone, Mirella ; Cespugli, Raymond ; Marinesco, Stephane
Author_Institution :
CNRS UPR 9040, Gif-sur-Yvette
Abstract :
D-serine is a glial neuromodulator that is linked to several brain disorders such as schizophrenia, epilepsy, or stroke. We have developed an in situ D-serine microbiosensor that consists in a cylindrical platinum microelectrode covered with a layer of D-amino acid oxidase from the yeast R. gracilis. This enzyme converts D-serine into hydroxypyruvate, while producing hydrogen peroxide that, in turn, is oxidized by the electrode. Interferences by biogenic amines or ascorbic acid are greatly reduced by an additional membrane of poly-m-phenylene diamine. Our microbiosensor can monitor D-serine levels in vivo with a high temporal and spatial resolution. This method will make it possible to study D- serine dynamics in the central nervous system.
Keywords :
biomedical electrodes; biosensors; enzymes; microsensors; D-amino acid oxidase microbiosensor; D-serine detection; ascorbic acid; biogenic amine; central nervous system; cylindrical platinum microelectrode; hydrogen peroxide; hydroxypyruvate; neuromodulator; poly-m-phenylene diamine; yeast R. gracilis; Biochemistry; Biomembranes; Central nervous system; Electrodes; Epilepsy; Fungi; Interference; Microelectrodes; Monitoring; Platinum; amperometry; brain; electrochemical detection; enzymatic biosensor; microelectrode;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International
Conference_Location :
Lyon
Print_ISBN :
1-4244-0842-3
Electronic_ISBN :
1-4244-0842-3
DOI :
10.1109/SENSOR.2007.4300081
