Phototransistor-Based Optoelectronic Tweezers for Cell Manipulation in Highly Conductive Solution

Author

Hsu, H.Y. ; Ohta, A.T. ; Chiou, P.Y. ; Jamshidi, A. ; Wu, M.C.

Author_Institution

Berkeley Sensor & Actuator Center, Berkeley

fYear

2007

fDate

10-14 June 2007

Firstpage

477

Lastpage

480

Abstract

We report on a novel phototransistor-based optoelectronic tweezers (OET) that, for the first time, enables operation in high-conductivity solutions, such as physiological buffers and cell culture media. This new capability allows the manipulation of cells in biocompatible environments. The controlled transport of HeLa and Jurkat cells at velocities of up to 35 mum/s in phosphate-buffered saline (PBS) solution has been demonstrated.

Keywords

biomedical engineering; cellular biophysics; optoelectronic devices; phototransistors; HeLa cells; Jurkat cells; cell culture media; cell manipulation; highly conductive solution; phosphate-buffered saline solution; phototransistor-based optoelectronic tweezers; physiological buffers; Actuators; Biomedical optical imaging; Cells (biology); Dielectrophoresis; Impedance; Optical buffering; Optical control; Optical sensors; Photoconducting devices; Photoconductivity; Optoelectronic tweezers; cell manipulation; dielectrophoresis;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International

Conference_Location

Lyon

Print_ISBN

1-4244-0842-3

Electronic_ISBN

1-4244-0842-3

Type

conf

DOI

10.1109/SENSOR.2007.4300171

Filename

4300171