Title :
Meniscus-Assisted Magnetic Bead Trapping on Ewod-Based Digital Microfluidics for Specific Protein Localization
Author :
Shah, Gaurav J. ; Pierstorff, Erik ; Ho, Dean ; Kim, Chang-Jin C J
Author_Institution :
UCLA, Los Angeles
Abstract :
This paper presents a technique to localize specific proteins utilizing magnetic beads (MBs) in digital (droplet) microfluidics, operated by electrowetting-on-dielectric (EWOD). We identify the fundamental differences between digital and continuous microfluidics in utilizing magnetic beads, and demonstrate the technique on an EWOD device. We also show specific, localized concentration of a target protein over antibody- conjugated MBs ("MB-Abs") as proof of concept.
Keywords :
bioMEMS; biomagnetism; magnetic materials; microfluidics; EWOD-based digital microfluidics; antibody-conjugated MB; digital droplet microfluidics; electrowetting-on-dielectric; meniscus-assisted magnetic bead trapping; protein localization; Aerospace engineering; Force sensors; Magnetic devices; Magnetic fields; Magnetic flux; Magnetic forces; Magnetic separation; Microfluidics; Permanent magnets; Protein engineering;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International
Conference_Location :
Lyon
Print_ISBN :
1-4244-0842-3
Electronic_ISBN :
1-4244-0842-3
DOI :
10.1109/SENSOR.2007.4300228
