DocumentCode
3374516
Title
Development of a Cellular Biochip Mimicking in Vitro Organs for Chronic Toxicity Analysis
Author
Baudoin, Régis ; Griscom, Laurent ; Corlu, Anne ; Layrolle, Pierre ; Legallais, Cécile ; Leclerc, Eric
Author_Institution
Univ. de Technol. de Compiegne, Compiegne
fYear
2007
fDate
10-14 June 2007
Firstpage
795
Lastpage
798
Abstract
MDCK, HepG2/C3a, HepaRG and bone marrow cells were used as liver, kidney and bone model respectively inside a PDMS biochip. Healthy culture conditions during 96 hours, including 72 hours of perfusion were demonstrated by the cell proliferation (MDCK, HepG2) and basic metabolism activities (HepaRG, bone marrow). An example of chronic toxicity was performed by using ammonia chloride loadings that have reduced the proliferation of the MDCK.
Keywords
ammonium compounds; biomimetics; bone; cellular biophysics; kidney; liver; microfluidics; tissue engineering; toxicology; HepG2/C3a; HepaRG; MDCK; PDMS biochip; ammonia chloride loadings; bone; bone marrow cells; cell culture; cell proliferation; cellular biochip mimicking; chronic toxicity analysis; kidney; liver; metabolism activities; In vitro; in vitro toxicology; microfluidic; polydimethylsiloxane cell biochips; tissue engineering;
fLanguage
English
Publisher
ieee
Conference_Titel
Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International
Conference_Location
Lyon
Print_ISBN
1-4244-0842-3
Electronic_ISBN
1-4244-0842-3
Type
conf
DOI
10.1109/SENSOR.2007.4300250
Filename
4300250
Link To Document