Title :
Development of a Cellular Biochip Mimicking in Vitro Organs for Chronic Toxicity Analysis
Author :
Baudoin, Régis ; Griscom, Laurent ; Corlu, Anne ; Layrolle, Pierre ; Legallais, Cécile ; Leclerc, Eric
Author_Institution :
Univ. de Technol. de Compiegne, Compiegne
Abstract :
MDCK, HepG2/C3a, HepaRG and bone marrow cells were used as liver, kidney and bone model respectively inside a PDMS biochip. Healthy culture conditions during 96 hours, including 72 hours of perfusion were demonstrated by the cell proliferation (MDCK, HepG2) and basic metabolism activities (HepaRG, bone marrow). An example of chronic toxicity was performed by using ammonia chloride loadings that have reduced the proliferation of the MDCK.
Keywords :
ammonium compounds; biomimetics; bone; cellular biophysics; kidney; liver; microfluidics; tissue engineering; toxicology; HepG2/C3a; HepaRG; MDCK; PDMS biochip; ammonia chloride loadings; bone; bone marrow cells; cell culture; cell proliferation; cellular biochip mimicking; chronic toxicity analysis; kidney; liver; metabolism activities; In vitro; in vitro toxicology; microfluidic; polydimethylsiloxane cell biochips; tissue engineering;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International
Conference_Location :
Lyon
Print_ISBN :
1-4244-0842-3
Electronic_ISBN :
1-4244-0842-3
DOI :
10.1109/SENSOR.2007.4300250
