Novel Optoelectronic Platform using an Amorphous/Nanocrystalline Silicon Biosensor for the Specific Identification of Unamplified Nucleic Acid Sequences Based on Gold Nanoparticle Probes

Author

Silva, L.B. ; Baptista, P. ; Raniero, L. ; Doria, G. ; Franco, R. ; Martins, R. ; Fortunato, E.

Author_Institution

Univ. Nova de Lisboa, Caparica

fYear

2007

fDate

10-14 June 2007

Firstpage

935

Lastpage

938

Abstract

Here we describe an innovative optoelectronic platform which enables the specific detection of unamplified nucleic acid sequences with the integration of oligonucleotide-derivatized gold nanoparticles, a colour sensor and a light emission source for a colorimetric detection method. This new low cost, fast and simple optoelectronic platform permits detection of less than 1 picomole quantities of nucleic acid without target or signal amplification.

Keywords

DNA; bio-optics; biosensors; colorimetry; molecular biophysics; nanostructured materials; optoelectronic devices; amorphous-nanocrystalline silicon biosensor; colorimetric detection method; colour sensor; oligonucleotide-derivatized gold nanoparticles; optoelectronic platform; unamplified nucleic acid sequences; Amorphous materials; Biosensors; DNA; Gold; Nanoparticles; Optoelectronic and photonic sensors; Probes; RNA; Sequences; Silicon; Nucleic acids; amorphous/nanocrystalline silicon sensor; gold nanoparticles; optoelectronic;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International

Conference_Location

Lyon

Print_ISBN

1-4244-0842-3

Electronic_ISBN

1-4244-0842-3

Type

conf

DOI

10.1109/SENSOR.2007.4300285

Filename

4300285