Biosensor for the Detection of Specific DNA Sequences by Impedance Spectroscopy

Author

Berdat, Daniel ; Sauvage, V. ; Bernau, L. ; Killis, D. ; Gijs, M.A.M.

Author_Institution

Ecole Polytech. Fed. de Lausanne, Lausanne

fYear

2007

fDate

10-14 June 2007

Firstpage

951

Lastpage

954

Abstract

In the agro-food industry there is a great need for rapid, sensitive and label-free biosensors for food quality control, for example the detection of pathogenic agents such as listeria or salmonella in dairy food samples. We are currently working on the development of a real-time monitoring system based on an interdigitated electrode DNA biochip for the detection of specific DNA target sequences. The biosensor contains four active areas on which DNA probes of various kinds can be immobilized, and is integrated into a microfluidic system. Impedimetric measurements of matching and non-matching synthetic target DNA sequences have been performed.

Keywords

biocomputing; computerised monitoring; microfluidics; software agents; biosensor; food quality control; interdigitated electrode DNA biochip; microfluidic system; pathogenic agents; real-time monitoring system; specific DNA sequence detection; Biosensors; DNA; Electrical equipment industry; Electrochemical impedance spectroscopy; Food industry; Industrial control; Pathogens; Quality control; Real time systems; Sequences; Biosensor; DNA immobilization; impedance spectroscopy; photolithography;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International

Conference_Location

Lyon

Print_ISBN

1-4244-0842-3

Electronic_ISBN

1-4244-0842-3

Type

conf

DOI

10.1109/SENSOR.2007.4300289

Filename

4300289