A Robust Fluorescence Multiplex Immunoassay Biosensor Designed for Field Applications

Author

Schultz, E. ; Perraut, F. ; Flahaut, T. ; Planat-Chretien, A. ; Galland, R. ; Lesbre, F. ; Hoang, A. ; Boutal, H. ; Volland, H.

Author_Institution

Lab. d´Imagerie et des Syst. d´Acquisition, Grenoble

fYear

2007

fDate

10-14 June 2007

Firstpage

955

Lastpage

958

Abstract

A novel portable fluorescence-based biosensor designed for field applications has been developed and successfully applied to the determination of two analytes, a neuropeptide model Substance P, and a small toxin Aflatoxin BI. A competitive immunoassay format called SPIT-FRI for Solid-Phase Immobilized Tripod for Fluorescent Renewable Immunoassay is used. The simplicity and robustness of the system apparatus is shown. Results clearly illustrate the assets of the developed array biosensor, by allowing in a portable instrument (i) simple regeneration steps, (ii) real-time monitoring, and (iii) multiplex detection thanks to a localized and cumulative fluorescence measurement.

Keywords

biosensors; computerised monitoring; fluorescence; multiplexing equipment; biosensor array; cumulative fluorescence measurement; field applications; fluorescent renewable immunoassay; real-time monitoring; robust fluorescence multiplex immunoassay biosensor; solid-phase immobilized tripod; Biomedical optical imaging; Biosensors; Fluorescence; Immune system; Instruments; Monitoring; Optical reflection; Optical sensors; Optical surface waves; Robustness; immunoassay optical biosensor; real-time detection; surface reaction monitoring; total internal reflection;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International

Conference_Location

Lyon

Print_ISBN

1-4244-0842-3

Electronic_ISBN

1-4244-0842-3

Type

conf

DOI

10.1109/SENSOR.2007.4300290

Filename

4300290