Chitosan-Mediated BioMEMS Platform for Optical Sensing

Author

Badilita, V. ; Shamim, I. ; Yi, H. ; Koev, S.T. ; Gerasopoulos, K. ; Ghodssi, R.

Author_Institution

Univ. of Maryland, College Park

fYear

2007

fDate

10-14 June 2007

Firstpage

1095

Lastpage

1098

Abstract

We present an optical BioMEMS sensor with chitosan-mediated, spatially-selective functionalization of a microfluidic channel sidewall with single stranded probe DNA (ssDNA). High-contrast optical sensing of hybridization using integrated optical waveguides and fluorescently-tagged matching versus mismatching DNA sequences is demonstrated. The collected peak emission signal corresponding to matching DNA sequences (4 muM) exceeds the peak emission signal corresponding to mismatching DNA sequences (8 muM) by a factor of 50. We demonstrate that the device is robust and reusable by exposing it to two successive cycles of denaturation/re-hybridization, each time recovering the initial sensitivity and specificity.

Keywords

DNA; bioMEMS; biosensors; integrated optics; microsensors; optical sensors; optical waveguides; chitosan-mediated bioMEMS; fluorescently-tagged matching; integrated optical waveguides; microfluidic channel sidewall; optical bioMEMS sensor; optical sensing; peak emission signal; single stranded probe DNA; spatially-selective functionalization; Biomedical optical imaging; Biosensors; DNA; Integrated optics; Microfluidics; Optical sensors; Optical waveguides; Probes; Sequences; Stimulated emission; Chitosan; DNA hybridization; Integrated waveguides; Microfluidics;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International

Conference_Location

Lyon

Print_ISBN

1-4244-0842-3

Electronic_ISBN

1-4244-0842-3

Type

conf

DOI

10.1109/SENSOR.2007.4300325

Filename

4300325