Title :
Chitosan-Mediated BioMEMS Platform for Optical Sensing
Author :
Badilita, V. ; Shamim, I. ; Yi, H. ; Koev, S.T. ; Gerasopoulos, K. ; Ghodssi, R.
Author_Institution :
Univ. of Maryland, College Park
Abstract :
We present an optical BioMEMS sensor with chitosan-mediated, spatially-selective functionalization of a microfluidic channel sidewall with single stranded probe DNA (ssDNA). High-contrast optical sensing of hybridization using integrated optical waveguides and fluorescently-tagged matching versus mismatching DNA sequences is demonstrated. The collected peak emission signal corresponding to matching DNA sequences (4 muM) exceeds the peak emission signal corresponding to mismatching DNA sequences (8 muM) by a factor of 50. We demonstrate that the device is robust and reusable by exposing it to two successive cycles of denaturation/re-hybridization, each time recovering the initial sensitivity and specificity.
Keywords :
DNA; bioMEMS; biosensors; integrated optics; microsensors; optical sensors; optical waveguides; chitosan-mediated bioMEMS; fluorescently-tagged matching; integrated optical waveguides; microfluidic channel sidewall; optical bioMEMS sensor; optical sensing; peak emission signal; single stranded probe DNA; spatially-selective functionalization; Biomedical optical imaging; Biosensors; DNA; Integrated optics; Microfluidics; Optical sensors; Optical waveguides; Probes; Sequences; Stimulated emission; Chitosan; DNA hybridization; Integrated waveguides; Microfluidics;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International
Conference_Location :
Lyon
Print_ISBN :
1-4244-0842-3
Electronic_ISBN :
1-4244-0842-3
DOI :
10.1109/SENSOR.2007.4300325
