Title :
A Fully Functional, Packaged Active Microscaffold System with Fluid Perfusion and Electrical Stimulation/Recording Functionalities for 3-D Neuronal Culture Studies
Author :
Rowe, L. ; Choi, Y. ; Ross, J. ; Lee, K. ; Fogleman, N. ; Brewer, G.J. ; Vukasinovic, J. ; Glezer, A. ; DeWeerth, S.P. ; Frazier, A.B.
Author_Institution :
Georgia Inst. of Technol., Atlanta
Abstract :
This paper and presentation, for the first time, demonstrates a fully functional, packaged active microscaffold system with fluid perfusion and electrical stimulation/recording functionalities for 3-D neuronal culture studies. The fluid perfusion capabilities of the active microscaffold serve as an artificial circulatory system to enable 3-D growth and proliferation of re-aggregate neuronal cultures. Increased cell survival on microscaffolds with nutrient perfusion at 14 and 21 days in vitro (DIV) is presented. Additionally, initial characterization of the electrical stimulation/recording functionality of the microscaffold system is presented.
Keywords :
bioMEMS; bioelectric phenomena; biomedical engineering; biomedical measurement; cellular biophysics; haemorheology; microelectrodes; microfluidics; neurophysiology; patient treatment; 3-D neuronal culture studies; 3-D neuronal growth; artificial circulatory system; bioMEMS; cell survival; electrical recording functionalities; electrical stimulation; fluid perfusion; in vitro analysis; microelectrodes; microfluidics; packaged active microscaffold system; Biological neural networks; Biomedical engineering; Circulatory system; Electrical stimulation; Electrodes; Gold; In vitro; Neurons; Packaging; Sensor arrays; BioMEMS; SU-8; microelectrodes; microfluidics; microscaffold;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International
Conference_Location :
Lyon
Print_ISBN :
1-4244-0842-3
Electronic_ISBN :
1-4244-0842-3
DOI :
10.1109/SENSOR.2007.4300402
