"Smart" Microfluidic Preconcentration of Specific Biomolecules

We present a device for selective extraction, concentration and release of metabolic molecules using ribonucleic acid (RNA) aptamers. The device consists of microfabricated PDMS channels on a glass substrate including a micro-chamber packed with an aptamer stationary phase. Features include: extraction and preconcentration of trace amounts of adenosine monophosphate (model analyte); sufficiently low temperature release and regeneration at 32.5degC (safe for thermally sensitive target analytes and functionalized surfaces); and extensive reuse. In addition, aptamer is active in aqueous solution eliminating the required use of potentially harsh solvents and reagents.