Title :
Voltammetric Analysis of DNA Films Immobilized on Gold Film Electrodes Carrying Single Base Pair Mismatches
Author :
Pänke, Oliver ; Lisdat, Fred
Author_Institution :
Univ. of Appl. Sci. Wildau, Wildau
Abstract :
The application of electrochemical techniques for DNA detection is motivated by their potential to detect hybridisation events in a more rapid, simplistic and cost-effective manner compared to conventional optical assays. Here, we analyzed DNA films immobilized onto gold film electrodes containing single base pair mismatches and covalently attached methylene blue (MB) by means of cyclic (CV) and differential pulse voltammetry (DPV) and determined the rate constant for the electron transfer reaction between MB moiety and electrode for different types of mismatches.
Keywords :
DNA; biosensors; electrodes; voltammetry (chemical analysis); DNA films; DNA hybridisation; MB moiety; biosensor; covalently attached methylene blue; cyclic voltammetry; differential pulse voltammetry; electrochemical DNA detection; electrochemical techniques; electron transfer reaction; gold film electrodes; hybridisation events; optical assays; single base pair mismatches; voltammetric analysis; Actuators; Biomedical electrodes; Biomedical engineering; DNA; Electrons; Event detection; Gold; Optical films; Sequences; Solid state circuits; Biosensor; DNA hybridisation; differential pulse voltammetry; electrochemical DNA detection; methylene blue; single base pair mismatch;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International
Conference_Location :
Lyon
Print_ISBN :
1-4244-0842-3
Electronic_ISBN :
1-4244-0842-3
DOI :
10.1109/SENSOR.2007.4300552
