Differential Mach-Zehnder Interferometer for Simultaneously Determining Size and Refractive Index of Living Cells

Author

Song, W.Z. ; Lim, C.S. ; Yap, P.H. ; Liu, A.Q.

Author_Institution

Nanyang Technol. Univ., Singapore

fYear

2007

fDate

10-14 June 2007

Firstpage

2159

Lastpage

2162

Abstract

This paper represents a differential method for simultaneously measuring the refractive index (RI) and the size of single living cells using the fiber-based Mach-Zehnder interferometer and optical trapping; both are integrated onto an optofluidic chip. In experiment, a living cancerous cell MDCK is trapped by 45-mW laser light and then the cell´s refractive index and diameter are simultaneously determined by the spectral shift in response to the buffer modulation and cell presence/absence. The measured cell has a size of 15.1 mum and a RI of 1.397 at an accuracy of 0.1%.

Keywords

Mach-Zehnder interferometers; biomedical measurement; cellular biophysics; laser applications in medicine; radiation pressure; refractive index measurement; size measurement; buffer modulation; cell absence; cell presence; differential Mach-Zehnder interferometer; fiber-based Mach-Zehnder interferometer; living cancerous cell MDCK; optical trapping; optofluidic chip; power 45 mW; refractive index measurement; size measurement; spectral shift; Charge carrier processes; Integrated optics; Optical buffering; Optical interferometry; Optical modulation; Optical refraction; Optical variables control; Refractive index; Semiconductor device measurement; Size measurement; Mach-Zehnder interferometer; living cells; optofluidics; refractive index;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International

Conference_Location

Lyon

Print_ISBN

1-4244-0842-3

Electronic_ISBN

1-4244-0842-3

Type

conf

DOI

10.1109/SENSOR.2007.4300594

Filename

4300594