Title :
The preliminary study of lactate detection based on lactate dehydrogenase/nictotinamide adenine dinucleotide
Author :
Taertulakarn, S. ; Tuantranont, Adisorn ; Tobanluepop, P. ; Pintavirooj, Chuchart
Author_Institution :
Fac. of Allied Health Sci., Thammasat Univ., Pathumthani, Thailand
Abstract :
This study reports the preliminary development to biosensor using electrochemistry for lactate analysis. In this work, PEDOT/PSS modified screen printed carbon was developed as working electrode with lactate dehydrogenase (LDH), the oxidized form of nicotinamide adenine dinucleotide (NAD+) and glutaraldehyde. The result shows that the current from working electrode with PEDOT/PSS were higher compared with screen printed carbon electrode. These results were consistent with PEDOT/PSS properties that are conducting polymers and can help for more rapid electron transfer. These favorable characteristics allowed its application to detect normal blood lactate concentration in human beings.
Keywords :
amperometric sensors; biochemistry; biosensors; conducting polymers; electrochemical electrodes; electrochemistry; enzymes; hydrogenation; molecular biophysics; oxidation; PEDOT-PSS modified screen printed carbon; PEDOT-PSS properties; biosensor; blood lactate concentration; conducting polymers; electrochemistry; electron transfer; glutaraldehyde; lactate analysis; lactate dehydrogenase-nictotinamide adenine dinucleotide; lactate detection; oxidation; screen printed carbon electrode; Biochemistry; Biosensors; Blood; Carbon; Electrodes; Substrates; Amperometric lactate biosensor; Lactate dehydrogenase; Lactate detection;
Conference_Titel :
Biomedical Engineering International Conference (BMEiCON), 2012
Conference_Location :
Ubon Ratchathani
Print_ISBN :
978-1-4673-4890-4
DOI :
10.1109/BMEiCon.2012.6465514
