Title :
A Microfluidic Oxygenator for Biological Cell Culture
Author :
Lam, Raymond H W ; Kim, Min-Cheol ; Thorsen, Todd
Author_Institution :
Massachusetts Inst. of Technol., Cambridge
Abstract :
In this paper, we report on the development of a microfluidic platform in which dissolved oxygen (DO) concentrations can be dynamically adjusted for cell culture applications. The DO concentration in culture media flowing through enclosed microchannels is regulated by the diffusion through an elastromeric interface from an overhead gas channel, using an oxygen-sensitive dye embedded in the fluid channel as a sensor. Using a microchannel network functioning as an oxygen/nitrogen mixer, the growth characteristics of murine fibroblast cells (3T3) were analyzed as a function of eight discrete DO concentrations in culture media within a single device, ranging from anaerobic (0 ppm) to fully saturated (42 ppm).
Keywords :
bioMEMS; cellular biophysics; diffusion; microchannel flow; biological cell culture; dissolved oxygen concentrations; elastromeric interface; fluid channel; microchannel network; microchannels; microfluidic oxygenator; murine fibroblast cells; overhead gas channel; oxygen-sensitive dye; oxygen/nitrogen mixer; Biological cells; Biosensors; Cells (biology); Filters; Gas detectors; Microchannel; Microfluidics; Nitrogen; Optical sensors; Sensor phenomena and characterization; cell; microfluid; oxygenator; sensor;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International
Conference_Location :
Lyon
Print_ISBN :
1-4244-0842-3
Electronic_ISBN :
1-4244-0842-3
DOI :
10.1109/SENSOR.2007.4300676
