Cloning and association analysis with cold adaptation of NADH-quinone oxidoreductase 3 subunit gene from common carp Cyprinus carpio

Genes expressed specifically at cooling temperature were used to design primers and to screen brain full-length cDNA library of common carp (Cyprinus carpio) through two rounds of clonal PCR method. After that, gene 152 was discovered and sequenced, and determined finally as NADH-quinone oxidoreductase 3 subunit gene using homologous searches. This gene was confirmed to play an important role as the force generators for fish in a cooling water environment. In addition, NADH-quinone oxidoreductase 3 subunit gene was associated with cold adaptation of common carp to some extent in oxidation of respiratory chain electron transport system.