Title :
Membrane chromatography for therapeutic DNA production: Adsorption membranes development
Author :
Mateus, Marcio ; Raiado-Pereira, Luis ; Prazeres, Miguel
Author_Institution :
Dept. of Bioeng., Inst. Super. Tecnico, Lisbon, Portugal
Abstract :
DNA vaccines and gene therapy have the potential to overcome several clinical pathologies such as infectious diseases and cancer. The active pharmaceutical ingredient in these therapeutics is plasmid DNA. This work describes the approach taken to develop and characterize adsorption membranes that can be used for membrane chromatography, boosting DNA therapeutics purification by better separation efficiency, increased solute selectivity and improved productivity. The membranes described herein were functionalized with alkyl or oleoyl ligands and these membranes were chemically and operationally characterized and compared with a commercial recently develop membrane for immunoglobulins purification.
Keywords :
DNA; adsorption; biochemistry; biomembranes; cellular biophysics; chromatography; gene therapy; molecular biophysics; proteins; purification; DNA therapeutics purification boosting; DNA vaccines; adsorption membranes; alkyl ligands; cancer; clinical pathologies; gene therapy; immunoglobulins; infectious diseases; membrane chromatography; oleoyl ligands; plasmid DNA; separation efficiency; solute selectivity; therapeutic DNA production; Biomembranes; Chemicals; DNA; Lipidomics; Purification; RNA; Vaccines; DNA vaccines; Gene Therapy; Membrane Chromatography; Plasmids Purification;
Conference_Titel :
Bioengineering (ENBENG), 2011. ENBENG 2011. 1st Portuguese Meeting in
Conference_Location :
Lisbon
Print_ISBN :
978-1-4577-0522-9
Electronic_ISBN :
978-1-4577-0521-2
DOI :
10.1109/ENBENG.2011.6026054
