Title :
Dimethyl 3,3´-dithiobis (propionimidate)-a novel crosslinking agent for collagen
Author :
Charulatha, V. ; Rajaram, A.
Author_Institution :
Biophys. Lab., Central Leather Res. Inst., Madras, India
Abstract :
Dimethyl 3,3´-dithiobis (propionimidate) [DTBP] has been examined as a crosslinking agent for collagen and the optimal conditions for crosslinking has been standardised. Crosslinking with DTBP introduces thermally stable intermolecular crosslinks and the crosslinked collagen was highly resistant to degradation by collagenase. The tensile strength of the material increases fourfold by optimal DTBP crosslinking. A comparison of the negative staining pattern of the DTBP and GTA treated collagen reveals that DTBP does not alter the banding pattern of collagen while GTA drastically alters it. Thus DTBP can be considered as an alternate crosslinking agent for collagenous matrices
Keywords :
biomechanics; molecular biophysics; organic compounds; proteins; tensile strength; collagen banding pattern; collagen crosslinking agent; collagenase; collagenous matrices; dimethyl 3,3´-dithiobis; negative staining pattern; optimal crosslinking conditions; propionimidate; thermally stable intermolecular crosslinks; Amino acids; Biophysics; Chemical analysis; Immune system; Laboratories; Optical fiber testing; Temperature; Thermal degradation; Thermal resistance; Wounds;
Conference_Titel :
Biomedical Engineering Conference, 1996., Proceedings of the 1996 Fifteenth Southern
Conference_Location :
Dayton, OH
Print_ISBN :
0-7803-3131-1
DOI :
10.1109/SBEC.1996.493221
