Dimethyl 3,3´-dithiobis (propionimidate)-a novel crosslinking agent for collagen

Author

Charulatha, V. ; Rajaram, A.

Author_Institution

Biophys. Lab., Central Leather Res. Inst., Madras, India

fYear

1996

fDate

29-31 Mar 1996

Firstpage

344

Lastpage

347

Abstract

Dimethyl 3,3´-dithiobis (propionimidate) [DTBP] has been examined as a crosslinking agent for collagen and the optimal conditions for crosslinking has been standardised. Crosslinking with DTBP introduces thermally stable intermolecular crosslinks and the crosslinked collagen was highly resistant to degradation by collagenase. The tensile strength of the material increases fourfold by optimal DTBP crosslinking. A comparison of the negative staining pattern of the DTBP and GTA treated collagen reveals that DTBP does not alter the banding pattern of collagen while GTA drastically alters it. Thus DTBP can be considered as an alternate crosslinking agent for collagenous matrices

Keywords

biomechanics; molecular biophysics; organic compounds; proteins; tensile strength; collagen banding pattern; collagen crosslinking agent; collagenase; collagenous matrices; dimethyl 3,3´-dithiobis; negative staining pattern; optimal crosslinking conditions; propionimidate; thermally stable intermolecular crosslinks; Amino acids; Biophysics; Chemical analysis; Immune system; Laboratories; Optical fiber testing; Temperature; Thermal degradation; Thermal resistance; Wounds;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering Conference, 1996., Proceedings of the 1996 Fifteenth Southern

Conference_Location

Dayton, OH

Print_ISBN

0-7803-3131-1

Type

conf

DOI

10.1109/SBEC.1996.493221

Filename

493221