Keratinocyte-seeded BIOBRANE for grafting of full thickness wounds

Author

Kopp, J. ; Bannasch, H. ; Andree, C. ; Voigt, M. ; Horch, R. ; Stark, G.B.

Author_Institution

Sect. of Plastic Surg., Freiburg Univ., Germany

fYear

1996

fDate

29-31 Mar 1996

Firstpage

545

Lastpage

547

Abstract

BIOBRANE an adherent, flexible temporary wound dressing was incubated with cultured human keratinocytes. The cells adhered quickly forming, “membrane-cell-grafts” (MCG) which were transplanted on full thickness wounds created on athymic mice. Healing was observed and immunohistochemistry, immunofluorescence and electron microscopy were performed. Conventional cultured epidermal grafts (CEG) served as control. Compared with CEG-grafted sites a reduced wound contraction was noticed and complete remodeling of the basement membrane zone was found. The efficiency of the easy, uncomplicated production and use as well as the short culture period could lead to a new approach in the treatment of burn and chronic wounds

Keywords

cellular biophysics; prosthetics; skin; adherent flexible temporary wound dressing; athymic mice; basement membrane zone remodeling; burn patients treatment; chronic wounds; cultured human keratinocytes; electron microscopy; full thickness wounds grafting; healing; immunofluorescence; immunohistochemistry; keratinocyte-seeded BIOBRANE; short culture period; wound contraction; Biomembranes; Cells (biology); Humans; Immune system; In vitro; Mice; Protection; Skin; Surgery; Wounds;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering Conference, 1996., Proceedings of the 1996 Fifteenth Southern

Conference_Location

Dayton, OH

Print_ISBN

0-7803-3131-1

Type

conf

DOI

10.1109/SBEC.1996.493298

Filename

493298