Title :
Application of computerized image analysis in quantitative microscopy
Author :
Flotte, Thomas J.
Author_Institution :
Wellman Lab., Massachusetts Gen. Hospital, Harvard Med. Sch., Boston, MA, USA
Abstract :
Digitized images obtained through a light microscope permit quantification of measured parameters. The author has been studying the use of video densitometry to analyze volume fractions of elastic tissue in skin, the localization of B-carotene in atherosclerosis by special ratioing of absorbances at different wavelengths, and the quantification of fluorophores. The application of these techniques of video densitometry to diagnostic pathology offers the possibility of obtaining quantitative information which will be reproducible from laboratory to laboratory; however, these studies must be analyzed with an understanding of the hardware and techniques.<>
Keywords :
computerised picture processing; densitometry; medical computing; optical microscopy; patient diagnosis; skin; B-carotene; atherosclerosis; computerized image analysis; diagnostic pathology; elastic tissue; fluorophores; light microscope; microscopy; skin; video densitometry;
Conference_Titel :
Engineering in Medicine and Biology Society, 1988. Proceedings of the Annual International Conference of the IEEE
Conference_Location :
New Orleans, LA, USA
Print_ISBN :
0-7803-0785-2
DOI :
10.1109/IEMBS.1988.94530
